 CHROMO Domain | |
|
|
Structure:
 The Chromo domain appears as an N-terminal three-stranded anti-parallel b-sheet which folds against an N-terminal a-helix. A conserved series of hydrophobic residues that winds across the face of the b-sheet is referred to as a sash. Interactions with partner proteins are thought to be mediated by the residues within the hydrophobic sash. The figure shows the structure of the chromo domain from mouse modifier protein 1.
Structure Reference:
Jacobs, SA. et al. (2002) Science. 295(5562): 2080-3. PDB: 1KNA.
|
|
Domain binding and function:
The Chromatin Organization Modifier (Chromo) domain is defined as a 30–70 amino acid residue protein module found in a number of proteins involved in the assembly of protein complexes on chromatin. This domain was first described in Drosophila modifiers of variegation proteins that modify the structure of chromatin to the condensed morphology of heterochromatin, a cytologically visible region within which gene expression is repressed. Examples of chromo-domain-containing proteins include the HP1 the Polycomb (Pc) transcriptional repressors and the human retinoblastoma binding protein (RBP-1). Chromo domains function to promote binding to methylated lysines in the tail region of Histone H3. Chromo domains can function individually or in tandem such as CHD1 to recognize specific methylated Histone tails.
| |
|
Examples of Proteins:
| CROMO domain protein |
|
Binding partner |
| HP1 |  |
Histone H3 methylated lysine-9 |
Polycomb Proteins
|
| Histone H3 methylated lysine-27
|
CHD1
|
|
Histone H3 methylated lysine-4
|
|
|
|
| |
 |
|
Referenced in part on Cell Signaling Technology Website, Reference Section on Protein Domains. We gratefully acknowledge the following contributors:
Piers Nash1, Dan Lin3, Kathleen Binns2, Clark Wells2, Rob Ingham2, Terry Kubiseski2, Bernard Liu1, Matt Smith2,3, Ivan Blasutig2,3, Maria Sierra1, Caesar Lim2,3, Michael Arc1, Jim Fawcett2 and Tony Pawson2,3.
1. Ben May Institute for Cancer Research, The University of Chicago, Chicago, Illinois, 60637, USA
2. Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
3. Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada
| |
|
|
Last Updated ( Monday, 15 October 2007 )
|
