 TPR Domain | |
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Structure:
 TPR motifs exhibit a large degree of sequence diversity. However, structural comparison reveals a highly conserved three dimensional structure. Individual TPR domains are composed of two anti-parallel alpha helices separated by a turn. Multiple TPR domains arrange at regular angles and form a right-handed superhelix. This creates a groove with a large amount of surface area available for ligand binding.
Structure Reference:
Scheufler, C. et al. (2000) Cell. 101(2): 199-210. PDB: 1ELW.
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Domain binding and function:
The tetratricopeptide repeat (TPR) motif was originally identified in yeast as a protein-protein interaction module in cell cycle proteins. It has since been found in organisms ranging from bacteria to humans. The TPR motif is a degenerate sequence of ~34 amino acids loosely based around the consensus residues -W-LG-Y-A-F-A-P-. The sequence occurs in tandem arrays and is present in over 800 different proteins. TPR motif-containing proteins act as scaffolds for the assembly of different multiprotein complexes including the anaphase promoting, the peroxisomal import receptor and the NADPH oxidase complexes.
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Examples of Proteins:
| TPR Domain Proteins |
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Binding Partners |
Peptide Ligands |
| PEX5 |  |
PTS-1 target signal |
S-K-L-COOH |
| Hop | |
Hsp70 - C-term heptapeptide
Hsp90 - C- term pentapeptide |
E-E-V-D-COOH
E-E-V-D-COOH |
| p67phox | |
GTP-Rac |
surface contacts |
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Referenced in part on Cell Signaling Technology Website, Reference Section on Protein Domains. We gratefully acknowledge the following contributors:
Piers Nash1, Dan Lin3, Kathleen Binns2, Clark Wells2, Rob Ingham2, Terry Kubiseski2, Bernard Liu1, Matt Smith2,3, Ivan Blasutig2,3, Maria Sierra1, Caesar Lim2,3, Michael Arc1, Jim Fawcett2 and Tony Pawson2,3.
1. Ben May Institute for Cancer Research, The University of Chicago, Chicago, Illinois, 60637, USA
2. Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
3. Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada
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