 PAS Domain | |
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Structure:
 The PAS domains consists of a conserved α/β-fold with no obvious conservation in amino acid sequence. The common fold of PAS domains is characterized by several α-helices flanking a five-stranded anti-parallel β-sheet. There are a few conserved residues that form part of the hydrophobic core located around αA. The loops at the αA/αB segment is important for ligand binding. Structure Reference:Morais Cabral, J.H. et al. (1998) Cell 95(5), 649-655. Erbel, PJ. et al. (2003) PNAS. 100(26): 15504-15509. PDB: 1P97.
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Domain binding and function:
PAS (Per-ARNT-Sim) domains are modules found in all Kingdoms, first identified in the Drosophila protein PER and ARNT. The PAS domains consists of a conserved α/β-fold with no obvious conservation in amino acid sequence. PAS domains have important roles as sensory modules for oxygen tension, redox potential or light intensities. The domain functions through protein-protein interactions or through binding cofactors within their hydrophobic cores to regulate protein-protein interactions in response to stimuli. For HIFα, in response to hypoxic conditions, the PAS-B domain heterodimerizes with PAS-B of ARNT which is mediated by the basic helix-loop-helix leading to transcriptional activation. The PAS domain for PASK, is a regulatory sensor that has a compound and ligand binding region that initiates a switch for regulating the kinase activity.
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Examples of Proteins: | PAS domain proteins |
| Binding partner
| Consensus binding motif
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| HIFα |  |
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| | ARNT (HIFβ) | |
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| | PASK | |
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| | HERG | |
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| NCoA-1
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| STAT6
| LXXLL
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| Referenced in part on Cell Signaling Technology Website, Reference Section on Protein Domains. We gratefully acknowledge the following contributors: Piers Nash1, Dan Lin3, Kathleen Binns2, Clark Wells2, Rob Ingham2, Terry Kubiseski2, Bernard Liu1, Matt Smith2,3, Ivan Blasutig2,3, Maria Sierra1, Caesar Lim2,3, Michael Arc1, Jim Fawcett2 and Tony Pawson2,3.
1. Ben May Institute for Cancer Research, The University of Chicago, Chicago, Illinois, 60637, USA
2. Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
3. Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada | |
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