Home Contact Us News Links   Top Module Empty Main Menu Home Research Publications People & Contact Info Lab photos Facilities Links Search e-services - Internet Domains - Map Domains - Listed Reagents and Protocols Technology Overview NetworKIN NetPhorest Domain Club Browser CoreFlow Analysis Mgmt Network Biology Centre eTOCs/Podcasts Featured Paper Bookmark Us Home eTOCs/Podcasts eTOCs/Podcasts Nature Methods - Issue - nature.com science feeds Nature Methods is a science methodology journal publishing laboratory techniques and methods papers in the life sciences and areas of chemistry relevant to the life sciences. Preprints in biology We remind our readers about our policies on the use of preprints: in short, we support them. A Nature Methods author can post a preprint prior to submission without fearing a penalty. The Author File: Sarah Teichmann Team sports and team efforts lead to a new tool that helps with immune profiling. Points of Significance: Analyzing outliers: influential or nuisance? Some outliers influence the regression fit more than others. iCOBRA: open, reproducible, standardized and live method benchmarking Synthetic biology: Customizing cell-cell communication Synthetic Notch receptors with chimeric extra- and intracellular domains can be tailored to tune cell-cell communication. Structural biology: Breaking the diffraction barrier Researchers exploit crystal imperfections to solve protein structures at resolutions beyond the X-ray diffraction limit. Genomics: Cas9 and the importance of asymmetry Short single-stranded DNA donors that asymmetrically span the Cas9 cut site show high efficiency in homology-directed editing. Sensors and probes: Yes to genetically encoded NO? sensors Researchers have developed a set of fluorescent-protein-based sensors for endogenous nitric oxide. Chemical biology: Expanding incorporation of nonstandard amino acids Stem cells: Cellular reprogramming is not very mutagenic Imaging: Clearing the way for RNA visualization Synthetic biology: Printing human-scale tissues in three dimensions Sensors and probes: Brighter and more photostable red and green fluorescent proteins Chemical biology: A super-splicing split intein Neuroscience: A transformative tool for trans-synaptic tracing Sensors and probes: Improved protein labeling for super-resolution microscopy Systems biology: Protein isoforms: more than meets the eye Alternative splicing imparts distinct functions through isoform-specific protein?protein interactions. Stem cells: Reprogrammed cells leave their past lives behind Genetic background trumps tissue of origin as a source of variability between induced pluripotent stem cell lines, diminishing the role of somatic memory in reprogrammed cells. Cancer: hunting rare somatic mutations Emerging ways to lower the error rate when hunting low-frequency mutations. Overcoming obstacles in localization microscopy Lattice light-sheet-PAINT brings localization microscopy to large volumes, and SuReSim enables ground truth modeling of super-resolution experiments. Analysis of computational footprinting methods for DNase sequencing experiments This comparison of ten computational methods for detecting transcription factor binding sites in DNase hypersensitive regions in the genome determines which methods work consistently well, how DNase-seq experimental artifacts should be corrected for and which score is best for ranking methods. Inferring causal molecular networks: empirical assessment through a community-based effort The HPN-DREAM community challenge assessed the ability of computational methods to infer causal molecular networks, focusing specifically on the task of inferring causal protein signaling networks in cancer cell lines. SuReSim: simulating localization microscopy experiments from ground truth models SuReSim software simulates single-molecule localization microscopy data for structures with known ground truth models to facilitate proper design, interpretation and validation of super-resolution imaging experiments. epiGBS: reference-free reduced representation bisulfite sequencing EpiGBS (epi-genotyping by sequencing) constructs de novo references using reduced representation bisulfite sequencing followed by variant calling and methylation detection. Simultaneous fast measurement of circuit dynamics at multiple sites across the mammalian brain Frame-projected independent-fiber photometry (FIP) enables the concurrent monitoring and manipulation of neural activity at multiple sites in the brains of freely behaving mice. T cell fate and clonality inference from single-cell transcriptomes The TraCeR tool extracts full-length, paired T cell receptor sequences from single-cell RNA-sequencing data from T lymphocytes, enabling a combination of clonotype and functional analysis. High-resolution mass spectrometry of small molecules bound to membrane proteins A high-resolution, Orbitrap-based, native mass spectrometry approach allows the direct characterization of lipid, peptide and drug binding to intact membrane proteins. Sensory and optogenetically driven single-vessel fMRI High-field fMRI with single-vessel resolution allows one to decipher the contribution of different types of vessels to hemodynamic activity evoked by sensory or optogenetic stimulation in the rat brain. Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond timescale This report describes an imaging and analysis platform enabling high-throughput single-molecule fluorescence investigations of fast, transient molecular recognition events. A saposin-lipoprotein nanoparticle system for membrane proteins A saposin protein?lipid nanoparticle system stabilizes diverse, fragile membrane proteins in a lipid environment for structural and functional studies. Apollo-NADP+: a spectrally tunable family of genetically encoded sensors for NADP+ HomoFRET sensors based on G6PD homodimerization allow direct sensing of NADPH/NADP+ redox state in living cells. Spectrally tuning the Apollo-NADP+ sensor allows multiplex imaging for studies of oxidative stress in beta cells. High-density three-dimensional localization microscopy across large volumes Lattice light-sheet and PAINT microscopy are combined to achieve low-background detection of dense molecular labels, yielding super-resolution localization microscopy images of intricate 3D structures within dividing cells and embryos. Tissue-specific regulatory circuits reveal variable modular perturbations across complex diseases This resource contains 394 human cell type? and tissue-specific transcriptional networks and finds that disease-associated genetic variants often perturb regulatory modules in tissues specific for that disease. One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy The SWAp-Tag method allows for easy replacement of a tag in a parental strain with an expression cassette of choice to rapidly create new yeast libraries that will enable researchers to address biological questions in Saccharomyces cerevisiae. Corrigendum: The field that came in from the cold Performing efficient sample preparation with hard tumor tissue: Precellys® bead-beating homogenizer solution [ Back ]

© 2016 The Pawson Lab

Joomla! is Free Software released under the GNU/GPL License.