 GAT Domain | |
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| Class:Ubiquitin processes | |
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Structure:
 The N-GAT subdomain or hook subdomain is comprised of a helix-loop-helix structure where the N-terminal half of the second long helix α1 is responsible for ARF binding. The C-GAT subdomain constitutes a 3-helix bundle composed of the C-terminal half of α1, α2 and α3. Binding of ubiquitin is mediated by the interaction between residues on one side of α3 helix of the GAT domain and the Ile-44 surface patch of ubiquitin.
Structure Reference:
Collins, B.M. et al. (2003) Dev. Cell. 4(3): 321-332.
Suer, S. et al. (2003) PNAS . 100(8): 4451-4456.
Shiba, T. et al. (2003) Nat. Struc. Biol. 10(5): 386-392. PDB: 1O3X.
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Domain binding and function:
The GAT (GGA and Tom1) domains are known to interact with GTP-bound ARF and are crucial for membrane recruitment of GGAs to the trans-Golgi network. Results have found the GAT domain of GGAs to possess dual functions. The N-terminal subdomain of GAT is bound to GTP-ARF as the C-terminal subdomain is bound to ubiquitin. The GAT domain is found in 25 proteins from various species and contains a three-helix bundle with a hydrophobic core. Both GGAs and Tom1 are capable of binding ubiquitin via their GAT domains suggesting roles for these proteins in mediating intracellular trafficking and sorting.
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Examples of Proteins:
| GAT domain protein |
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Binding partner |
| GGA1 |  |
ARF1
,ARF3
,Rabaptin-5
,Ubiquitin
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| Tom1 | |
Tollip
,Ubiquitin
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Referenced in part on Cell Signaling Technology Website, Reference Section on Protein Domains. We gratefully acknowledge the following contributors:
Piers Nash1, Dan Lin3, Kathleen Binns2, Clark Wells2, Rob Ingham2, Terry Kubiseski2, Bernard Liu1, Matt Smith2,3, Ivan Blasutig2,3, Maria Sierra1, Caesar Lim2,3, Michael Arc1, Jim Fawcett2 and Tony Pawson2,3.
1. Ben May Institute for Cancer Research, The University of Chicago, Chicago, Illinois, 60637, USA
2. Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada
3. Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada
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