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Efficient T-cell receptor signaling Print E-mail
Written by Seet, B.   
Wednesday, 02 August 2006

Efficient T-cell receptor signaling requires a high-affinity interaction

between the Gads C-SH3 domain and the SLP-76 RxxK motif.

Seet BT, Berry DM, Maltzman JS, Shabasson J, Raina M, Koretzky GA, McGlade

CJ, Pawson T.

EMBO J.  2007 Feb 7;26(3):678-89.

PMID: 17235283

DOI: 10.1038/sj.emboj.7601535

The relationship between the binding affinity and specificity of modular interaction domains is potentially important in determining biological signaling responses. In signaling from the T-cell receptor (TCR), the Gads C-terminal SH3 domain binds a core RxxK sequence motif in the SLP-76 scaffold. We show that residues surrounding this motif are largely optimized for binding the Gads C-SH3 domain resulting in a high-affinity interaction (K(D)=8-20 nM) that is essential for efficient TCR signaling in Jurkat T cells, since Gads-mediated signaling declines with decreasing affinity. Furthermore, the SLP-76 RxxK motif has evolved a very high specificity for the Gads C-SH3 domain. However, TCR signaling in Jurkat cells is tolerant of potential SLP-76 crossreactivity, provided that very high-affinity binding to the Gads C-SH3 domain is maintained. These data provide a quantitative argument that the affinity of the Gads C-SH3 domain for SLP-76 is physiologically important and suggest that the integrity of TCR signaling in vivo is sustained both by strong selection of SLP-76 for the Gads C-SH3 domain and by a capacity to buffer intrinsic crossreactivity.



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