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Tuesday, 02 September 2008 |
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Created with both in vitro and in vivo data, NetPhorest
is an atlas of consensus sequence motifs for 179 kinases and 104
phosphorylation-dependent binding domains and reveals new insight into
phosphorylation-dependent signaling. [PAPER]
NetPhorest is a community resource that uses phylogenetic trees to
organize data from both in vivo and in vitro experiments to derive
sequence specificities for 179 kinases and 104 domains (SH2, PTB, BRCT,
WW, and 14–3–3) that bind to phosphorylated sites. The resulting atlas
of linear motifs revealed that oncogenic kinases tend to be less
specific in the target sequences they phosphorylate than their
non-oncogenic counterparts, that autophosphorylation sites tend to be
more variable than other substrates of a given kinase, and that
coupling interaction domains with kinase domains may allow
phosphorylation site specificity to be low while still maintaining
substrate specificity.
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Last Updated ( Friday, 05 September 2008 )
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