Top Module Empty

Bookmark Us

Print E-mail
IQ Domain

No ImageThe IQ domain forms an uninterrupted seven-turn α-helix that is distinctly amphiphilic. The non-polar face of the helix switches sides after the fifth turn, providing an additional surface where contacts with ligand can occur. Residues important for interactions with calmodulin include a hydrophobic amino acid at position 1, a highly conserved glutamine at position 2, basic charges at positions 6 and 11, and a variable glycine at position 7. The substitution of this glycine with residues possessing bulky side chains, which occurs in approximately 50% of IQ domains, may provide some specificity for calmodulin interactions, as might the identity of the 6 intervening amino acids in the IQ consensus sequence.

Structure Reference:
Terrak, M. et al. (2002) Acta Crystallogr D Biol Crystallogr. 58(Pt10 Pt 2): 1882-5. PDB: 1N2D.

Domain binding and function:
The IQ domain is approximately 25 amino acids in length and is widely distributed in nature.  The motif conforms to the consensus sequence [I,L,V]QxxxRGxxx[R,K],  which forms an amphiphilic seven-turn α-helix capable of binding calmodulin in a Ca2+-independent manner.  IQ motifs result in different light chain conformations: compact and extended.  Calmodulin mediates the effects of the major cellular second signal Ca2+, and can stimulate changes in the actin cytoskeleton through proteins such as myosin.  Nearly all myosins possess between one and seven IQ domains, with most being found in multiple tandem repeats separated by 9-16 residues.  Binding of calmodulin by IQ motifs from a diverse set of proteins might allow Ca2+ signaling to effect a wide range of cellular activities.  Proteins found to contain at least one IQ domain include myosins, voltage-operated channels, several neuronal growth proteins, phosphatases, sperm surface proteins, Ras exchange proteins, spindle-associated proteins, a RasGAP-like protein and several plant-specific proteins.
Examples of Proteins: 
IQ domain proteins
Binding partners
Myo2p (class V myosin) Mlc1p (calmodulin-like myosin light chain), Ca2+-independent
Neurogranin CaM (calmodulin), Ca2+-independent and regulated by PKC phosphorylation of the IQ motif
Ras-GRF (exchange factor) CaM (calmodulin), Ca2+-induced binding to IQ domain

< Prev   Next >
© 2016 The Pawson Lab
Joomla! is Free Software released under the GNU/GPL License.